{"id":2963,"date":"2020-09-28T17:17:44","date_gmt":"2020-09-28T15:17:44","guid":{"rendered":"https:\/\/www.elysia-bioscience.com\/services\/targeted-approach\/pcr\/"},"modified":"2020-10-02T10:21:09","modified_gmt":"2020-10-02T08:21:09","slug":"pcr","status":"publish","type":"page","link":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/","title":{"rendered":"PCR"},"content":{"rendered":"\n
\"\"<\/figure>
\n

Quantitative PCR<\/h2>\n\n\n\n

RT-qPCR (quantitative PCR or real-time PCR) is a technique for accurately measuring the expression of genes of interest.<\/p><\/p>\n\n\n\n

<\/div>\n\n\n\n

Indeed, PCR allows the amplification of a DNA sequence, which may initially be present in minute quantities, to generate millions of copies, based on the natural process of DNA replication. <\/p>\n<\/div><\/div>\n\n

<\/div>\n\n
\"\"<\/figure>
\n

Quantification of genes of interest<\/h2>\n\n\n\n
<\/div>\n\n\n\n

For instance, you can quantify the expression of the numerous genes coding for proteins whose job is to manage oxidative stress. By doing so, you will observe the behavior of the target genes under conditions of oxidative stress. To detect significant variations, the expression of the target genes is compared to at least two reference genes.<\/p>\n\n\n\n

<\/p>\n<\/div><\/div>\n\n

<\/div>\n\n
\"\"<\/figure>
\n

Mitochondrial DNA assay<\/h2>\n\n\n\n
<\/div>\n\n\n\n

Did you know, mitochondria contain their own DNA, called mitochondrial DNA. And so, mitochondrial DNA can be used to estimate the abundance of mitochondria (mitochondrial mass) present in a cell. And since mitochondria allow the production of energy<\/a>, the mitochondrial mass is a good indicator of cellular well-being.<\/p><\/p>\n<\/div><\/div>\n\n

<\/div>\n\n
\n
\n
<\/div>
\n

Applications<\/h3>\n\n\n\n
  • Assess mitochondrial mass by the mitochondrial DNA assay<\/li>
  • Detail the mechanism of action by RNA targeting<\/li>
  • Obtain detailed scientific data<\/li><\/ul><\/div><\/section>\n<\/div>\n\n\n\n
    \n
    <\/div>
    \n

    Advantages<\/h3>\n\n\n\n
    • Fast analysis<\/li>
    • Powerful and sensitive tool<\/li>
    • Specific to the desired target<\/li>
    • Quantitative evaluation of target DNA or RNA<\/li><\/ul><\/div><\/section>\n<\/div>\n<\/div>\n\n
      <\/div>\n","protected":false},"excerpt":{"rendered":"

      Measure the influence of your product on gene expression.<\/p>\n","protected":false},"author":2,"featured_media":2073,"parent":2938,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"","ocean_second_sidebar":"","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"","ocean_custom_header_template":"","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"","ocean_menu_typo_font_family":"","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"","footnotes":""},"class_list":["post-2963","page","type-page","status-publish","has-post-thumbnail","hentry","entry","has-media"],"yoast_head":"\nPCR - Elysia Bioscience<\/title>\n<meta name=\"description\" content=\"PCR is one of the techniques that Elysia Bioscience implements to make your products safe for health and the environment.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"PCR - Elysia Bioscience\" \/>\n<meta property=\"og:description\" content=\"PCR is one of the techniques that Elysia Bioscience implements to make your products safe for health and the environment.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/\" \/>\n<meta property=\"og:site_name\" content=\"Elysia Bioscience\" \/>\n<meta property=\"article:modified_time\" content=\"2020-10-02T08:21:09+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"1707\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/\",\"url\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/\",\"name\":\"PCR - Elysia Bioscience\",\"isPartOf\":{\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg\",\"datePublished\":\"2020-09-28T15:17:44+00:00\",\"dateModified\":\"2020-10-02T08:21:09+00:00\",\"description\":\"PCR is one of the techniques that Elysia Bioscience implements to make your products safe for health and the environment.\",\"breadcrumb\":{\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#primaryimage\",\"url\":\"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg\",\"contentUrl\":\"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg\",\"width\":2560,\"height\":1707,\"caption\":\"Test tube with DNA on abstract background. 3d illustration\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.elysia-bioscience.com\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"R&D solutions\",\"item\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Targeted approach\",\"item\":\"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/\"},{\"@type\":\"ListItem\",\"position\":4,\"name\":\"PCR\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/#website\",\"url\":\"https:\/\/www.elysia-bioscience.com\/en\/\",\"name\":\"Elysia Bioscience\",\"description\":\"R\u00e9v\u00e9latrice du potentiel vivant\",\"publisher\":{\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.elysia-bioscience.com\/en\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/#organization\",\"name\":\"Elysia Bioscience\",\"url\":\"https:\/\/www.elysia-bioscience.com\/en\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/09\/logo-grand.png\",\"contentUrl\":\"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/09\/logo-grand.png\",\"width\":2002,\"height\":1127,\"caption\":\"Elysia Bioscience\"},\"image\":{\"@id\":\"https:\/\/www.elysia-bioscience.com\/en\/#\/schema\/logo\/image\/\"},\"sameAs\":[\"https:\/\/www.linkedin.com\/in\/emilie-obre-b4293492\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"PCR - Elysia Bioscience","description":"PCR is one of the techniques that Elysia Bioscience implements to make your products safe for health and the environment.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/","og_locale":"en_US","og_type":"article","og_title":"PCR - Elysia Bioscience","og_description":"PCR is one of the techniques that Elysia Bioscience implements to make your products safe for health and the environment.","og_url":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/","og_site_name":"Elysia Bioscience","article_modified_time":"2020-10-02T08:21:09+00:00","og_image":[{"width":2560,"height":1707,"url":"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/","url":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/","name":"PCR - Elysia Bioscience","isPartOf":{"@id":"https:\/\/www.elysia-bioscience.com\/en\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#primaryimage"},"image":{"@id":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#primaryimage"},"thumbnailUrl":"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg","datePublished":"2020-09-28T15:17:44+00:00","dateModified":"2020-10-02T08:21:09+00:00","description":"PCR is one of the techniques that Elysia Bioscience implements to make your products safe for health and the environment.","breadcrumb":{"@id":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#primaryimage","url":"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg","contentUrl":"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/08\/pcr-scaled.jpeg","width":2560,"height":1707,"caption":"Test tube with DNA on abstract background. 3d illustration"},{"@type":"BreadcrumbList","@id":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/pcr\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.elysia-bioscience.com\/en\/"},{"@type":"ListItem","position":2,"name":"R&D solutions","item":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/"},{"@type":"ListItem","position":3,"name":"Targeted approach","item":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/"},{"@type":"ListItem","position":4,"name":"PCR"}]},{"@type":"WebSite","@id":"https:\/\/www.elysia-bioscience.com\/en\/#website","url":"https:\/\/www.elysia-bioscience.com\/en\/","name":"Elysia Bioscience","description":"R\u00e9v\u00e9latrice du potentiel vivant","publisher":{"@id":"https:\/\/www.elysia-bioscience.com\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.elysia-bioscience.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.elysia-bioscience.com\/en\/#organization","name":"Elysia Bioscience","url":"https:\/\/www.elysia-bioscience.com\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.elysia-bioscience.com\/en\/#\/schema\/logo\/image\/","url":"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/09\/logo-grand.png","contentUrl":"https:\/\/www.elysia-bioscience.com\/wp-content\/uploads\/2020\/09\/logo-grand.png","width":2002,"height":1127,"caption":"Elysia Bioscience"},"image":{"@id":"https:\/\/www.elysia-bioscience.com\/en\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.linkedin.com\/in\/emilie-obre-b4293492\/"]}]}},"_links":{"self":[{"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/pages\/2963","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/comments?post=2963"}],"version-history":[{"count":3,"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/pages\/2963\/revisions"}],"predecessor-version":[{"id":3029,"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/pages\/2963\/revisions\/3029"}],"up":[{"embeddable":true,"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/pages\/2938"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/media\/2073"}],"wp:attachment":[{"href":"https:\/\/www.elysia-bioscience.com\/en\/wp-json\/wp\/v2\/media?parent=2963"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}