{"id":2969,"date":"2020-09-28T17:22:04","date_gmt":"2020-09-28T15:22:04","guid":{"rendered":"https:\/\/www.elysia-bioscience.com\/services\/targeted-approach\/western-blot\/"},"modified":"2020-10-02T10:37:20","modified_gmt":"2020-10-02T08:37:20","slug":"western-blot","status":"publish","type":"page","link":"https:\/\/www.elysia-bioscience.com\/en\/rd-solutions\/targeted-approach\/western-blot\/","title":{"rendered":"Western Blot"},"content":{"rendered":"\n
\"\"<\/figure>
\n

Precise protein analysis<\/h2>\n\n\n\n

The Western Blot<\/a> is an analytical method that detects and quantifies specific proteins in a protein mixture. The principle consists of separating proteins according to their molecular weight or charge and then applying labeled antibodies, to visualize the desired proteins.<\/p><\/p>\n\n\n\n

<\/div>\n\n\n\n

This reference technique can provide a detailed description of the mechanism of action of a product on a specific cellular pathway.<\/p><\/p>\n<\/div><\/div>\n\n

<\/div>\n\n
\"\"<\/figure>
\n

Specific protein quantification by Western Blot<\/h2>\n\n\n\n

Indeed, it is possible to assay one or more proteins belonging to the same cellular pathway to demonstrate the mechanism of action. Also, at Elysia Bioscience, normalization is not performed on a reference protein (e.g. actin) but on all proteins present, for a more realistic and accurate normalization<\/a>.<\/p><\/p>\n\n\n\n

<\/p>\n<\/div><\/div>\n\n

<\/div>\n\n
\"\"<\/figure>
\n

Oxyblot<\/h2>\n\n\n\n

Among its applications, the Western Blot technique can be used to reveal oxidized proteins. Indeed, oxidized proteins have a modification of their carbonyl groups. These modifications are then detected on a blot. <\/p>\n\n\n\n

Overall, the Oxyblot technique allows you to quickly explore the anti-oxidant power of a compound in a simple, visual, and quick way.<\/p><\/p>\n<\/div><\/div>\n\n

<\/div>\n\n
\n
\n
<\/div>
\n

Applications<\/h3>\n\n\n\n
  • Details of the mechanism of action by specific proteins<\/li>
  • Evaluate protein modifications (oxidation, phosphorylation)<\/li>
  • Obtain detailed scientific data<\/li><\/ul><\/div><\/section>\n\n\n\n

    <\/p>\n<\/div>\n\n\n\n

    \n
    <\/div>
    \n

    Advantages<\/h3>\n\n\n\n
    • Fast analysis<\/li>
    • Protein separation by size (sensitive and specific)<\/li>
    • Simultaneous detection of several targets<\/li>
    • Normalization to total amount of protein<\/li><\/ul><\/div><\/section>\n<\/div>\n<\/div>\n\n
      <\/div>\n","protected":false},"excerpt":{"rendered":"

      Test how the cell’s proteins respond to your product.<\/p>\n","protected":false},"author":2,"featured_media":2075,"parent":2938,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"","ocean_second_sidebar":"","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"","ocean_custom_header_template":"","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"","ocean_menu_typo_font_family":"","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"","footnotes":""},"class_list":["post-2969","page","type-page","status-publish","has-post-thumbnail","hentry","entry","has-media"],"yoast_head":"\nWestern Blot - 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